Tom Butler, U Washington Title: Inferring RNA Orientation during Translocation through a Biological Nanopore Abstract: We make single-molecule measurements of the electrophoretic translocation of single-stranded DNA and RNA through a biological ion channel. This system serves as a unique model in which to investigate various biologically relevant nanoscale physical processes. It is also hoped that this system can be developed into a novel, extremely fast DNA sequencing technology. I will give an overview of our apparatus and the general trends we observe in DNA/RNA translocation signals. I will then describe our most recent results concerning the orientation of RNA molecules during translocation.