Table 3: Volumes of substock and other solutions required to prepare standard curve.
________________________________________________________________________________
Amount       Amount  Amount     Amount    Absorbance
of substock  of pNP  of buffer  of NaOH   at 405 nm
[µl]        [nmols]   [µl]      [ml]
________________________________________________________________________________
0                      200         2
40                     160         2
80                     120         2
120                     80         2
160                     40         2
200                      0         2
________________________________________________________________________________
back