Fluorescence Activated Cell Sorting (FACS)

Technical Support

Contact

The FACS technician, Winnie Enns (J. Choy lab), is available for assistance with use of the instrument.

WINNIE ENNS
Research Staff - Choy Lab

Email: wenns@sfu.ca
Tel: (778) 782-8707

Location

TASC II 8130

FACS Booking Systems

Fee Schedule

Type

Fee

Cell Sorting                     
$80/hr
Data Acquisition and Analysis                              $40/hr           

FACS Equipment

BD Jazz

  • Data acquisition and cell sorting capabilities (2 tubes, 24, 36,...96-well plates, slides)
  • Lasers: 488 nm (blue), 640 nm (red)
  • Band pass filters
    • Blue laser: 530/30 nm (FITC), 585/29 nm (PE or PI), 692/40 nm (PerCP), 750/LP (PE-Cy7)
    • Red laser: 670/30 nm (APC), 750/LP (APC-Cy7)
  • Software: BDsortware for acquisition, FloJo for analysis
    • Data acquisition only
    • Lasers: 488 nm (Blue), 640 nm (Red)
    • Band pass filters: 525/30 nm, 583/26 nm, 690/50 nm, 660/19 nm, 785/70 nm

Millipore Guava

  • Data acquisition only
  • Lasers: 488 nm (Blue), 640 nm (Red)
  • Band pass filters: 525/30 nm, 583/26 nm, 690/50 nm, 660/19 nm, 785/70 nm

FACS Resources

Training and Standard Operating Procedures

Funding

Funding for the FACS Facility was initially faciliated through a Tier I Canada Research Chair (to J. Scott) and provided by the following National and B.C. agencies, and units at SFU: Canadian Foundation for Innovation, British Columbia Knowledge Development Fund, Molecular Biology and Biochemistry, Dean of Science, NSERC Research tools and Instruments grant (biosafety cabinet).