Spring 2017 - MBB 308 D100

Molecular Biology Laboratory (3)

Class Number: 1529

Delivery Method: In Person

Overview

  • Course Times + Location:

    Mo 2:30 PM – 4:20 PM
    WMC 3260, Burnaby

  • Prerequisites:

    MBB 331 with a minimum grade of C-

Description

CALENDAR DESCRIPTION:

Modern molecular biological and recombinant nucleic acid methods will be covered. Examples are DNA and RNA isolation, plasmid preparation, restriction enzyme digestion, DNA cloning and polymerase chain reaction. Students with credit for BISC 357 may not take this course for further credit.

COURSE DETAILS:

This course introduces students to recombinant DNA methods, and includes a two hour lecture plus one full afternoon of lab work per week.  You must attend all lab sessions because you and your partner will be relying upon each other, and because this component is worth 50% of your grade. There will be additional open lab time before and/or after, to setup and/or to analyze results.

Laboratory Exercises:

  1. Introduction: setting up a lab, safety, equipment, microbiology and sterile technique, using micropipettors.
  2. Preparation and characterization of genomic DNA.
  3. Plasmid miniprep, restriction digestion, gel electrophoresis.
  4. Polymerase Chain reaction.
  5. Isolation of DNA from a gel, subcloning into a plamid.
  6. Site-directed mutagenesis.
  7. DNA sequencing. Computer analysis of DNA sequence data.

Experiments are subject to change or rearrangement.

Grading

  • Lab reports, lab notebook, quizzes, performance in the lab (exact proportions TBA) 50%
  • 2 in-class exams 50%

NOTES:

No lab the first week of classes.

Materials

MATERIALS + SUPPLIES:

Safety goggles and a lab coat are required. 

A non-refundable fee of $12.50 will be assesssed for the laboratory manual.

RECOMMENDED READING:

Dale, Jeremy W. and Malcolm von Schantz. (2012).  From Genes to Genomes and Applications of DNA Technology, (3rd Ed.). Wiley.
ISBN: 9780470683859

Department Undergraduate Notes:

  • Students are advised to review the plagiarism tutorial found at
    http://www.lib.sfu.ca/help/tutorials/plagiarism-tutorial
  • For help with writing, learning and study strategies please contact the Student Learning Commons at
    http://learningcommons.sfu.ca/
  • Students requiring accommodations as a result of a disability, must contact the Centre for Students with Disabilities (778-782-3112 or e-mail:  csdo@sfu.ca)

Registrar Notes:

SFU’s Academic Integrity web site http://students.sfu.ca/academicintegrity.html is filled with information on what is meant by academic dishonesty, where you can find resources to help with your studies and the consequences of cheating.  Check out the site for more information and videos that help explain the issues in plain English.

Each student is responsible for his or her conduct as it affects the University community.  Academic dishonesty, in whatever form, is ultimately destructive of the values of the University. Furthermore, it is unfair and discouraging to the majority of students who pursue their studies honestly. Scholarly integrity is required of all members of the University. http://www.sfu.ca/policies/gazette/student/s10-01.html

ACADEMIC INTEGRITY: YOUR WORK, YOUR SUCCESS