Fall 2022 - BISC 357 D100
Genetic Engineering (4)
Class Number: 1623
Delivery Method: In Person
Course Times + Location:
Mo, We, Fr 10:30 AM – 11:20 AM
AQ 3005, Burnaby
Prerequisites:BISC 202, MBB 222, and MBB 231, all with a grade of C- or better. Recommended: MBB 331.
Techniques in gene cloning and the evaluation of gene function are introduced in lectures and practiced in labs. Lab exercises include genomic DNA, RNA, and plasmid purification; PCR; various cloning techniques; and construction of novel genes. Students are introduced to additional techniques in lectures including gene editing, and genome sequencing. Students with credit for MBB 308 may not take this course for further credit.
There is a demand for people that are skilled in the various techniques associated with genetic engineering. Biomedical companies and institutes regularly search for people with practical experience in genetic engineering. These skills are also useful for an academic career in disciplines ranging from medicine to ecology. First you will learn how to purify DNA and RNA and clone a complementary DNA fragment in a basic plasmid. After that, you will design and generate a DNA construct for CRISPR-based loss-of-function analysis of gene function, followed by Sequence and Ligase Independent Cloning for over expression of genes as well as marker gene-based evaluation of where and when genes are expressed in an organism. The course will also contain a brief introduction to key technologies behind the ongoing revolution in genomics research. Teaching and exams focus on the practical logic of experiments rather than extensive rote memorization. The course is housed on Canvas; we may use a supplementary website with additional resources.
- • Laboratory work (quizzes, lab notebook checks, worksheets, other assessments): 40%
- • Bioinformatics exercises: 10%
- • Midterm Exam: 20%
- • Final Exam: 30%
Course is being updated, grading distribution subject to minor tweaks.
Mode of Teaching
Midterm(s): synchronous; date: TBA
Final exam: synchronous; date: TBA
MATERIALS + SUPPLIES:
We will be using an online lab notebook platform called LabArchives which involves a small cost.
You will need a lab coat and protective eyewear for labs.
Lecture: Posted lecture notes, and online e-text
Lab: Provided protocols and lab handouts
Plus: Any supplementary on-line material and links that are provided
ACADEMIC INTEGRITY: YOUR WORK, YOUR SUCCESS
SFU’s Academic Integrity web site http://www.sfu.ca/students/academicintegrity.html is filled with information on what is meant by academic dishonesty, where you can find resources to help with your studies and the consequences of cheating. Check out the site for more information and videos that help explain the issues in plain English.
Each student is responsible for his or her conduct as it affects the University community. Academic dishonesty, in whatever form, is ultimately destructive of the values of the University. Furthermore, it is unfair and discouraging to the majority of students who pursue their studies honestly. Scholarly integrity is required of all members of the University. http://www.sfu.ca/policies/gazette/student/s10-01.html