Paetzel Lab - Simon Fraser University
Publications
Synthesis and characterization of the Arylomycin lipoglycopeptide antibiotics and the crystallographic analysis of their complex with signal peptidase.
Liu, J., Luo, C., Smith, P.A., Chin, J.K., Page, M.G., Paetzel, M., & Romesberg, F.E.
J. Am. Chem. Soc. 133, 17869-17877 (2011)
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Crystal structure of the β-barrel assembly machinery BamCD complex.
Kim, K.H., Aulakh, S. & Paetzel, M.
J. Biol. Chem. 286, 39116-39121 (2011)
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Crystallographic analysis of the C-terminal domain of the Escherichia coli lipoprotein BamC.
Kim, K.H., Aulakh, S., Tan, W. & Paetzel, M.
Acta Crystallographica F 67, 1350-1358 (2011)
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Crystal structure of cardiac troponin C regulatory domain in complex with cadmium and deoxycholic acid reveals novel conformation.
Li, A.Y., Lee, J., Borek, D., Otwinowski, Z., Tibbits, G.F., & Paetzel, M.
J. Mol. Biol. 413, 699-711 (2011)
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Crystal structure of a viral protease intramolecular acyl-enzyme complex. Insights into cis-cleavage at the VP4/VP3 junction of Tellina birnavirus.
Chung, I.Y. & Paetzel, M.
J. Biol. Chem. 286, 12475-12482 (2011)
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Structure of the catalytic domain of glucoamylase from Aspergillus niger.
Lee, J. & Paetzel, M.
Acta Crystallographica F 67, 188-192 (2011)
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Structural characterization of Escherichia coli BamE, a lipoprotein component of the β-barrel assembly machinery complex.
Kim, K.H., Kang, H.S., Okon, M., Escobar, E., McIntosh, L.P. & Paetzel, M.
Biochemistry 50, 1081-1090 (2011)
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Expression, purification and crystallization of VP4 protease from Tellina virus 1.
Chung, I.Y. & Paetzel, M.
Acta Crystallographica F 67, 157-160 (2011)
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Crystal structure of Escherichia coli BamB, a lipoprotein component of the β-barrel assembly machinery complex.
Kim, K.H. & Paetzel, M.
J. Mol. Biol. 406, 667-678 (2011)
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Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyl-transferase fold.
Lee, J., Johnson, J.E., Ding, Z., Paetzel, M. & Cornell, R.B.
J. Biol. Chem. 284, 33535-33548 (2009)
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Crystallographic analysis of bacterial signal peptidase in ternary complex with Arylomycin A2 and a β-sultam inhibitor.
Luo, C., Roussel, P., Dreier, J., Page, M.G., & Paetzel, M.
Biochemistry 48, 8976-8984 (2009)
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Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach.
Ekici,O.D., Zhu,J., Chung,I.Y., Paetzel, M., Dalbey, R.E. & Pei, D.
Biochemistry 48, 5753-5759 (2009)
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Structural analysis of a monomeric form of the twin-arginine leader peptide binding chaperone Escherichia coli DmsD.
Stevens,C.M, Winstone,T.M.L., Turner,R.J. & Paetzel, M.
J. Mol. Biol. 389, 124-133 (2009)
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Unconventional Serine Proteases: variations on the catalytic Ser/His/Asp triad configuration.
Ekici, O.D. Paetzel, M. & Dalbey, R.E.
Protein Science 17, 2023-2037 (2008)
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The Escherichia coli signal peptide peptidase a is a serine-lysine protease with a lysine recruited to the non-conserved amino-terminal domain in the S49 protease family.
Wang, P., Shim, E., Cravatt, B., Jacobsen, R., Schoeniger, J., Kim, A.C., Paetzel, M. & Dalbey, R.E.
Biochemistry 47, 6361-6369 (2008)
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Phage display and crystallographic analysis reveals potential substrate/binging site interactions in the protein secretion chaperone CsaA from Agrobacterium tumefaciens.
Feldman, A.R., Shapova, Y.A., Wu, S.S.T., Oliver, D.C., Heller, M., McIntosh, L.P., Scott, J.K, & Paetzel, M.
J. Mol. Biol. 379, 457-470 (2008)
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Identification of residues in DmsD for twin-arginine leader peptide binding, defined through random and bioinformatics-directed mutagenesis.
Chan, C.S., Winstone, T.M., Chang, L., Stevens, C.M., Workentine, M.L., Li, H., Wei, Y., Ondrechen, M.J., Paetzel, M., & Turner, R.J.
Biochemistry 47, 2749-2759 (2008)
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Crystal structure of a bacterial signal peptide peptidase.
Kim, A.C., Oliver, D.C., & Paetzel, M.
J. Mol. Biol. 376, 352-366 (2008)
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Crystal structure of the major periplasmic domain of the bacterial membrane protein assembly facilitator YidC.
Oliver, D.C., & Paetzel, M.
J. Biol. Chem. 283, 5208-5216 (2008)
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Crystal structure of the VP4 protease from infectious pancreatic necrosis virus reveals the acyl-enzyme complex for an intermolecular self-cleavage reaction.
Lee, J., Feldman, A.R., Delmas, B., & Paetzel, M.
J. Biol. Chem. 282, 24928-24937 (2007)
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Crystallographic analysis of Bacillus subtilis CsaA.
Shapova, Y.A. & Paetzel, M.
Acta Crystallographica D63, 478-485 (2007)
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Altered -3 substrate specificity of E. coli signal peptidase 1 mutants as revealed by screening a combinatorial peptide library.
Dogan Ekici, O., Karla, A., Paetzel, M., Lively, M.O., Pei, D., & Dalbey, R.E.
J. Biol. Chem.282, 417-425 (2007)
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Purification, crystallization and preliminary X-ray analysis of truncated and mutant forms of VP4 protease from infectious pancreatic necrosis virus.
Lee, J., Feldman, A.R., Chiu, E., Chan, C., Kim, Y.N., Delmas, B., & Paetzel, M.
Acta Crystallographica F62, 1235-1238 (2006)
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Crystal structure of a novel viral protease with a serine / lysine catalytic dyad mechanism.
Feldman , A. R., Lee, J., Delmas, B. & Paetzel, M.
J. Mol. Biol. 358, 1378-1389 (2006)
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Expression, purification and crystallization of a birnavirus-encoded protease, VP4, from blotched snakehead virus (BSNV).
Lee, J., Feldman , A. R., Delmas, B. & Paetzel, M.
Acta Crystallographica F62, 353-356 (2006)
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Membrane protein insertion in bacteria from a structural perspective.
Paetzel, M. & Dalbey, R. E.
Protein Movement Across Membranes, edited by Jerry Eichler, Eurika.com publishers (2005)
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The identification of residues that control signal peptidase cleavage fidelity and substrate specificity.
Karla, A., Lively, M., Paetzel, M. & Dalbey, R. E.
J. Biol. Chem. 280, 6731-6741 (2005)
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Novel avian influenza H7N3 strain outbreak, British Columbia.
Hirst, M., Astell, C. R., Griffith, M., Coughlin, S. M., Moksa, M., Zeng, T., Smailus, D. E., Holt, R. A., Jones, S., Marra, M. A., Petric, M., Krajden, M., Lawrence, D., Mak, A., Chow, R., Skowronski, D. M., Tweed, R. A., Goh, S., Brunham, R. C., Robinson, J., Bowes, V., Sojonky, K., Byrne, S. K., Li, Y., Kobasa, D., Booth, T., & Paetzel, M.
Emerging Infectious Diseases 10, 2192-2195 (2004)
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UmuD and UmuD' proteins.
Paetzel, M. and Woodgate R.
Handbook of Proteolytic Enzymes 2nd Edition Elsevier Ltd. ISBN 0-12-079610-4 pages: 1976-1981 (2004)
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Crystallographic and biophysical analysis of a bacterial signal peptidase in complex with a lipopeptide-based inhibitor.
Paetzel, M., Goodall, J.J., Kania, M., Dalbey, R.E. & Page, M.G.P.
J. Biol. Chem. 279, 30781-30790 (2004)
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Signal peptidases.
Paetzel, M., Karla, A., Strynadka, N.C.J. & Dalbey, R.E.
Chemical Reviews 102, 4549-4580 (2002)
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Crystal structure of a bacterial signal peptidase apo-enzyme: IMPLICATIONS FOR SIGNAL PEPTIDE BINDING AND THE SER-LYS DYAD MECHANISM.
Paetzel, M., Dalbey, R.E. & Strynadka, N.C.J.
J. Biol. Chem. 277, 9512-9519 (2002)
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Crystal structure of LexA: A conformational switch for regulation of self-cleavage.
Luo, Y., Pfuetzner, R.A., Mosimann, S., Paetzel, M., Frey, E.A., Cherney, M., Kim, B., Little, J.W., & Strynadka, N.C.J.
Cell 106, 585-594 (2001)
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Effect of divalent metal cations on the dimerization of OXA-10 and -14 Class D beta-lactamases from Pseudomonas aeruginosa.
Danel, F., Paetzel, M., Strynadka, N.C.J. & Page, M.G.P.
Biochemistry 40, 9412-9420 (2001)
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Characterization of AgfB from Salmonella enteritidis thin aggregative fimbriae.
White, A.P. Collinson, S.K., Banser, P.A, Gibson, D.L., Paetzel, M., Strynadka, N.C.J. & Kay, W.W.
J. Mol. Biol. 311, 735-749 (2001)
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Bacterial type I signal peptidases.
Carlos J.L., Paetzel, M., Klenotic, P.A., Strynadka, N.C.J. & Dalbey, R.E.
The Enzymes 3rd EditionVol. 22, 27-55 (2001)

Crystal structure of the class D beta-lactamase OXA-10.
Paetzel, M., Danel, F., de Castro, L., Mosimann, S.C., Page, M.G.P. & Strynadka, N.C.J.
Nature Structural Biology 7, 918-925 (2000)
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The role of the membrane spanning domain of type I signal peptidase in cleavage. Site Selection.
Carlos, J.L., Paetzel, M., Brubaker, G., Karla, A., Ashwell, C.M., Lively, M.O., Cao, G., Bullinger, P., Dalbey, R.E. ,
J. Biol. Chem. 275 , 38813-38822 (2000)
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The structure and mechanism of bacterial type I signal peptidases: a novel antibiotic target.
Paetzel, M., Dalbey, R.E., and Strynadka, N.C.J.,
Pharmacology & Therapeutics 87, 27-49 (2000)
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Mutational evidence of transition state stabilization by serine 88 in Escherichia coli type I signal peptidase.
Carlos, J.L., Klenotic, P.A., Paetzel, M., Strynadka, N.C.J., and Dalbey, R.E.
Biochemistry 39, 7276-7283 (2000)
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Structure-based design guides the improved efficacy of deacylation transition state analogue inhibitors of TEM-1 beta-lactamase.
Ness, S., Martin, R., Kindler, A.M., Paetzel, M., Gold, M., Jensen, S.E., Jones, J.B., and Strynadka, N.C.J.
Biochemistry 39, 5312-5321 (2000)
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The role of the conserved box E residues in the active site of the Escherichia coli type I signal peptidase.
Klenotic,P.A., Carlos, J.L., Samuelson, J.C., Schuenemann, T.A., Tschantz, W.R., Paetzel, M. Strynadka, N.C.J. and Dalbey, R.E.
J. Biol. Chem. 275, 6490-6498 (2000)
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Common protein architecture and binding sites in proteases utilizing a Ser/Lys dyad mechanism.
Paetzel, M. and Strynadka, N.C.J.
Protein Science 8, 2533-2536 (1999)
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Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor.
Paetzel, M., Dalbey,R.E., and Strynadka, N.C.J.
Nature 396, 186-190 (1998)
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Phosphatidylethanolamine mediates insertion of the catalytic domain of leader peptidase in membranes.
van Klompenburg, W., Paetzel, M., de Jong, J.M., Dalbey, R.E., Demel, R.A., von Heijne, G., and de Kruijff B.
Febs Letters 431, 75-79 (1998)
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Catalytic hydroxyl/amine dyads within serine proteases.
Paetzel, M. and Dalbey, R.E.
TIBS 22, 28-31 (1997)
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Use of site-directed chemical modification to study an essential lysine in Escherichia coli leader peptidase.
Paetzel, M., Strynadka, N.C.J., Tschantz, W.R., Casareno, R., Bullinger, P.R., and Dalbey, R.E.
J. Biol. Chem. 272, 9994-10003 (1997)
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Crystallization of a soluble, catalytically active form of Escherichia coli leader peptidase.
Paetzel, M., Chernaia, M., Strynadka, N., Tschantz, W., Cao, G., Dalbey, R.E., and James, M.N.G.
PROTEINS:Structure, Function, and Genetics 23, 122-125 (1995)
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Characterization of a soluble, catalytically active form of Escherichia coli leader peptidase:
requirement of detergent or phospholipid for optimal activity.
Tschantz, W.R., Paetzel, M., Cao, G., Suciu, D., Inouye, M., and Dalbey, R.E.
Biochemistry 34, 3935-3941 (1995)
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