Research in my laboratory has been directed towards understanding the genomic organization of a developmentally complex organism.
Over the past 20 years, we have been taking a forward genetic approach to identify and map genes using mutated genetic strains. As a consequence of these analyses, we identified nearly 500 essential genes. In parallel, we began investigating the evolutionary conservation of a related nematode. C. briggsae is almost indisguishable from C. elegans phenotypically, as assayed by the morphology of the rays in the male tail, a characteristic used to distinguish the two species. Our estimates suggest that separation of the two species occurred 23 - 40 million years ago. Although conservation of DNA sequences is largely confined to protein coding regions, conservation of short flanking sequences has been shown to correlate with promoter expression studies in the case of some genes.
For more details, visit our research lab website.
- The Distribution And Role Of Lethals (Essential Genes) In The C. Elegans Genome
- C. elegans Gene Expression Project (completed in 2006) - Members investigating C. elegans gene expression using promoter::GFP fusions and serial analysis of gene expression (SAGE)
- Cornes et al. Cytoplasmic LSM-1 protein regulates stress responses through the insulin/IGF-1 signaling pathway in Caenorhabditis elegans. RNA. 2015 Sep;21(9):1544-53. doi: 10.1261/rna.052324.115. Epub 2015 Jul 6. Read more
- Alriyami et al. let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans. Meta Gene. 2014 Nov 9;2:819-30. doi: 10.1016/j.mgene.2014.08.006. eCollection 2014 Dec. Read more
- Chu et al. High-throughput capturing and characterization of mutations in essential genes of Caenorhabditis elegans. BMC Genomics. 2014 May 12;15:361. doi: 10.1186/1471-2164-15-361. Read more