Our laboratory use quantitative microscopy approaches to study the role of mechanical forces, cytoskeleton and membrane dynamics in the innate immune response to microbial infections and cancer.
Fighting against infections and cancers is a physical task. Many steps of the immune responses require force generation by leukocytes: recruitment to the sites of inflammation, killing by phagocytosis or degranulation, migration to and from secondary lymphoid organs, antigen discrimination. Moreover, immune cell responses are largely affected by the mechanical properties of their tissue environment. Our lab works at the interface of immunology, cell biology, biophysics and microbiology. We are studying how immune cells generate and sense mechanical forces using quantitative approaches based on high-resolution live cell microscopy. We are particularly interested in molecular dynamics involved in plasma membrane-cytoskeleton interactions and how they impact immune functions of professional phagocytes.
- Walpole GFW, Plumb JP, Chung D, Tang B, Boulay B, Osborne DG, Piotrowski JT, Catz SD, Billadeau DD, Grinstein S and Jaumouillé V. Inactivation of Rho GTPases by Burkholderia cenocepacia induces a WASH-mediated actin polymerization that delays phagosome maturation. Cell Rep, 2020, 31:107721
- Jaumouillé V and Waterman CM. Physical constraints and forces involved in phagocytosis. Front Immunol, 2020, 11:1097
- Jaumouillé V, Cartagena-Rivera AX and Waterman CM. Coupling of β2 integrins to actin by a mechanosensitive molecular clutch drives complement receptor-mediated phagocytosis. Nat Cell Biol, 2019, 3: 1357-69
- Wong HS, Jaumouillé V, Freeman SA, Doodnauth SA, Schlam D, Canton J, Mukovozov IM, Saric A, Grinstein S and Robinson LA. Chemokine signaling enhances CD36 responsiveness towards oxidized low-density lipoproteins and accelerates foam cell formation. Cell Rep, 2016, 14: 2859-71
- Freeman SA, Jaumouillé V, Choi K, Hsu BE, Wong WS, Abraham L, Graves ML, Coombs D, Roskelley CD, Das R, Grinstein S and Gold MR. Toll-like receptor ligands sensitize B-cell receptor signaling by reducing actin-dependent spatial confinement of the receptor. Nat Commun, 2015, 6: 6168
- Jaumouillé V, Farkash Y, Jaqaman K, Das R, Lowell CA and Grinstein S. Actin cytoskeleton reorganization by Syk regulates Fcγ receptor responsiveness by increasing its lateral mobility and clustering. Dev Cell, 2014, 29: 534-46