Simon Fraser University
Engaging the World

Course Outlines

Fall 2023 - BPK 412 D100

Molecular Cardiac Physiology (3)

Class Number: 5081

Delivery Method: In Person

Overview

  • Course Times + Location:

    Sep 6 – Oct 6, 2023: Tue, 2:30–4:20 p.m.
    Burnaby

    Oct 11 – Dec 5, 2023: Tue, 2:30–4:20 p.m.
    Burnaby

  • Exam Times + Location:

    Dec 17, 2023
    Sun, 12:00–3:00 p.m.
    Burnaby

  • Prerequisites:

    BPK 305 or MBB 308.

Description

CALENDAR DESCRIPTION:

A detailed analysis of the molecular and cellular basis of cardiac function employing a multidisciplinary approach including structure (histology, ultrastructure, molecular), biophysics (electrophysiology and molecular/cellular biomechanics), physiology, biochemistry and cellular/molecular biology. Discussion of experimental techniques, including human induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs), will be used to examine the mechanisms by which inherited arrhythmias and cardiomyopathies manifest as a pathological phenotype.

COURSE DETAILS:

Course Canvas website: https://canvas.sfu.ca/courses

Lecture 1 / 2:      Tu 14:30 – 16:20           West Mall 2532

Tutorial 1:            Th 14:30 – 15:20           AQ5036                      Tutorial 2:                  Th 15:30 – 16:20     AQ5036

OVERVIEW

The course presents a detailed analysis of the mechanistic basis of cardiac function.  This is a multidisciplinary approach that includes anatomy (histology and ultrastructure), cellular and molecular biomechanics, physiology/biophysics, electrophysiology, biochemistry, and molecular biology.  There is an emphasis on cardiac contractile function, ion channels, channelopathies, inherited cardiac arrhythmias and cardiomyopathies.  Approaches include human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) and genome editing in their diagnosis and treatment.  Course structure: two hours of lecture and one-hour tutorial per week.

TUTORIAL

The tutorial serves several purposes.  First, it allows for the communication of new material that is best presented in a more Socratic style.  Second, questions and problems can be explored in greater detail.  Third, this is a forum for formal debates on controversial issues in the field.

 

Course Tutor Marker:                    Alia Arslanova

                                                  arslanov@sfu.ca

BRIEF COURSE OUTLINE

Cardiac Structure:

                                Anatomy, Histology, Ultrastructure

Cardiac Excitation

                                Electrophysiology, Channelopathies, Cardiac arrhythmias and ECG

                                Excitation-contraction coupling

Cardiac Contraction

                                Structure and function of cardiac contractile proteins

                                Cellular and molecular biomechanics

                                Hypertrophic Cardiomyopathies

Date

Time

L/T

Topic

Lecturer

Sept

07

 

L1

Course organization, overview of cardiac function

GFT

12

 

L2

Overview of cardiac excitation contraction coupling

GFT

12

 

L3

Cardiac contractile proteins: myosin

GFT

14

 

T1

Cardiac ultrastructure

GFT

19

 

L4

Regulation of myosin paralogs

GFT

19

 

L5

Cardiac contractile proteins: titin

GFT

21

 

T2

Molecular Techniques I – SDS Page, IB blots, Proteomics

GFT

26

 

L6

Cardiac contractile proteins: Myosin binding protein C

GFT

26

 

L7

Cardiac contractile proteins: troponin

GFT

28

 

T3

Molecular Techniques II – RT- PCR, Nanostring, RNAseq

GFT

Oct

03

 

L8

Familial hypertrophic cardiomyopathy

GFT

03

 

L9

Cardiac contractile proteins: thin filament

GFT

05

 

T4

Imaging Techniques I – Fluorescence microscopy, FRET

GFT

10

 

L10

Regulation of contractile protein sensitivity to Ca2+

GFT

10

 

 

Virtual Lecture

GFT

12

 

T5

Imaging Techniques II – Super Resolution Microscopy

GFT

17

 

 

Midterm Exam

 

GFT

17

 

 

19

 

T6

hiPSC-derived cardiomyocytes

GFT

24

 

L11

Ion channels in cardiac electrophysiology

TWC

24

 

L12

K+ channels: IK1 - Inward rectifiers

TWC

26

 

T7

Electrophysiological Techniques I

TWC

 

31

 

L13

K+ channels: IKs / KCNQ1

TWC

31

 

L14

K+ channels: IKr / hERG

TWC

Nov    02

 

T8

Electrophysiological Techniques II

TWC

07

 

L15

K+ channels: Ito and KV4.3

TWC

07

 

L16

K+ channels: pacemaking and HCN channels

TWC

09

 

T9

Electrophysiological virtual lab

TWC

14

 

L17

Ca2+ channels: Cardiac L-type Ca2+ channel

GFT

14

 

L18

Cardiac Ryanodine receptor – RyR2

GFT

16

 

T10

HR Regulation - Overview of Debate #1

GFT

21

 

L19

Ryanodine receptor – RyR - CPVT

GFT

21

 

L20

Cardiac NCX

GFT

23

 

T11

HCM - Overview of Debate # 2NCX

GFT

28

 

L21

NCX / SERCA2A

GFT

28

 

L22

SERCA2a

GFT

 

30

 

T12

CPVT – Overview of Debate

GFT

Dec    05

 

L23

Overview and review session (Room K9624 14:30 – 17:00)

GFT

 

 

 

 

 

 

 

 

 

 

 

 

 

Final Paper

GFT

 

 

 

 

 

 

 

 

 

GFT

 

 

 

 

 

 

Grading

  • Tutorial Participation (debate) 20%
  • Paper 20%
  • Midterm 20%
  • Final 40%

NOTES:

 

 

REQUIREMENTS:

PREREQUISITES

                BPK 305 (MBB 222 and 231 are highly recommended)

                                                                                                          = OR =

                MBB 308 (with instructor’s consent)

Materials

RECOMMENDED READING:

While there is no required text, the following texts will be on reserve in the library:

                Excitation-contraction coupling and cardiac contractile force                             QP 113.2 B47 2001

                         D.M. Bers, Kluwer Scientific Press (2001)                                                        ISBN: 0-7923-7158-5

Ion channels of excitable membranes (3rd Edition)                                              QH 603 I54 H54 2001

                         Bertil Hille, Sinauer Associates (2001)                                                              ISBN: 0-8789-3321-2

                Cardiac Electrophysiology: From Cell to Bedside (7th Edition)

  1. Zipes and J. Jaliffe, Saunders (2014) ISBN: 978-1-4557-2856-5

                         This e-book is available at: http://www.sciencedirect.com/science/book/9781455728565


REQUIRED READING NOTES:

Your personalized Course Material list, including digital and physical textbooks, are available through the SFU Bookstore website by simply entering your Computing ID at: shop.sfu.ca/course-materials/my-personalized-course-materials.

Department Undergraduate Notes:

It is the responsibility of the student to keep their BPK course outlines if they plan on furthering their education.

Registrar Notes:

ACADEMIC INTEGRITY: YOUR WORK, YOUR SUCCESS

SFU’s Academic Integrity website http://www.sfu.ca/students/academicintegrity.html is filled with information on what is meant by academic dishonesty, where you can find resources to help with your studies and the consequences of cheating. Check out the site for more information and videos that help explain the issues in plain English.

Each student is responsible for his or her conduct as it affects the university community. Academic dishonesty, in whatever form, is ultimately destructive of the values of the university. Furthermore, it is unfair and discouraging to the majority of students who pursue their studies honestly. Scholarly integrity is required of all members of the university. http://www.sfu.ca/policies/gazette/student/s10-01.html

RELIGIOUS ACCOMMODATION

Students with a faith background who may need accommodations during the semester are encouraged to assess their needs as soon as possible and review the Multifaith religious accommodations website. The page outlines ways they begin working toward an accommodation and ensure solutions can be reached in a timely fashion.