Positions Available in the Paetzel Lab
Graduate student positions (MSc or PhD)
Thesis projects vary from chemical to computational, from pathogen to host, and from basic research to applied research.
Your project will be designed to suit your interests and strengths.
Please send your CV, transcripts and cover letter to Dr. Mark Paetzel (mpaetzel@sfu.ca)
For information on graduate studies in MBB
Undergraduate directed research student positions
MBB 498-3, MBB 491-5, or MBB 481-5/482-5/483-5 options are available.
Wet lab or computer-base projects available.
Please send your CV, transcripts and cover letter to Dr. Mark Paetzel (mpaetzel@sfu.ca)
For information on Undergraduate Research Opportunities in MBB
The Paetzel lab can provide training and education in the following:
Molecular biology: Gene cloning, Construct design (fusions, deletions, mutations) Expression optimization (vector, host, temperature, media), Phage-display, Random mutagenesis libraries, Gene knock outs, Directed evolution.
Molecular isolation and purification: Cell lysis (chemical, sonication, pressure, osmotic shock), Differential centrifugation, Chromatography (affinity, ion-exchange, size-exclusion), Concentration techniques (semipermeable membranes - stir-cell, centrifuge, precipitation, lyophilization), Peptide purification via HPLC, Lipid analysis via TLC, Proteoliposome and other membrane mimetic preparations, Detergent micelle optimization, Inclusion body isolation, solubilization and refolding.
Molecular characterization: Electrophoresis (denaturing, native and IEF), Mass-spectrometry, Amino-acid chemical analysis, N-terminal sequencing, Concentration determination, determining oligomeric nature (analytical size-exclusion chromatography - light scattering), Enzyme kinetics, Kinetics of ligand binding (SPR, ITC), Stability (limited proteolysis, chemical and temperature titrations with intrinsic fluorescence, DSC).
Crystallography: Crystallization, Data collection and processing, Phasing (MR, MIR, MIRAS, SIR, SIRAS, MAD, SAD), Refinement, quality assessment and analysis.
Structural bioinformatics: Data base searching (PDB, UniProt), Archiving, Sequence and structural alignments, Accessible and buried surface area calculations, Electrostatic calculations, H-bond analysis, Ordered water analysis, Ligand binding interactions, Molecular docking, Molecular dynamics simulation calculations, Theoretical pKa calculations.
Scientific communication: Technical writing and figure making based on graphical design principles.
The Paetzel lab working environment:
The Paetzel lab strives to be a welcoming environment for all scholars regardless of their race, ethnicity, gender, age, religion, abilities/disabilities, sexual orientation, socioeconomic status, or country of origin.
Our commitment to diversity, equity and inclusion helps to enables collaborative and innovative research.