Databases:

Reverse-yeast-protein fasta with seven glycoproteins and common contaminants

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Protocols

Glycopeptide Capture for Cell Surface Proteomics

Video protocol




LC-MS System (available for service)



Q-Exactive HF

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A Hybrid Quadrupole-Orbitrap Mass Spectrometer

Equipped with an Ultrahigh-Field Orbitrap

Provide accurate and highly sensitive detection of peptides in complex biological samples

High throughput (> 1000 proteins/h) and high sensitivity (attomole)

Discovery based shotgun proteomics and targeted PRM analysis





Easy nLC 1000

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Deliver nanoliter per minute accurate and highly reproducible flow rate

Ideal for label free or PRM based analysis requring highly reproducible retension time in separation

Maxium pressure is 1000 bar suitable for columns with ultrafine resins of 3 nm or less

Ultrahigh resolution with peak with of a few seconds

Simple touch screen operation for both expert and nonexpert users





Easy-Spray source and LC columns

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Provide reliable and reproducible ionization

Ensure robust nano and capilary flow and separation

Simple plug and pull operation with reproducible position of the spray tip to the MS inlet

Temperature controlled column separation with digital thermostat

Integrated column/emitter design eliminates dead volume

NanoViper fitting allows tight seal for maximum 1000 bar operation pressure




Pricing

SFU rate $100/sample, Academic $150/sample, Corporate $250/sample (subject to change), Sample Submission Form




Mammalian Cell Culture Facility