To assist in the development of screens and to aid in identifying promising chemical matter we have started a small collection of literature references that users and potential users should find useful as a point of reference. The topics covered include:
- General parameters to consider when planning and developing HTS assays.
- Useful review for consideration during the planning and development of phenotypic screening assays.
- Uses of cell painting in phenotypic screening.
Note that hits obtained from high throughput screens are a starting point and require rigorous follow up assessment including evaluation of resynthesized compound, validation in a distinct secondary assay, and assessing the quality of the hit to rule out, for example, promiscuous electrophilic compounds or redox active compounds known as PAINS. Also reasonable common are hits that inhibit enzyme activity through compounds aggregating to form microscopic colloids that inhibit or inactivate compounds.
- For a review on problematic hits and ways to confirm the quality of hits.
- For a more specific discussion on the problem of PAINS and how to identify these.
- For a discussion on the problem of compound aggregators and how to identify these by prediction.
The authors thank the Centre for High-Throughput Chemical Biology (HTCB) at SFU for access to core facilities, which are supported by grants from the Glyconet Networks of Centres of Excellence, Canadian Foundation for Innovation (CFI), and British Columbia Knowledge Development Foundation (BCKDF).
The authors thank the Centre for High-Throughput Chemical Biology (HTCB) at SFU for access to core facilities.