Summer 2016 - MBB 308 D100
Molecular Biology Laboratory (3)
Class Number: 1621
Delivery Method: In Person
Course Times + Location:
May 9 – Aug 8, 2016: Mon, 2:30–4:20 p.m.
Prerequisites:MBB 231, and MBB 331 as a co- or pre-requisite (the latter is recommended), with a minimum grade of C.
Modern molecular biological and recombinant nucleic acid methods will be covered. Examples are DNA and RNA isolation, plasmid preparation, restriction enzyme digestion, DNA cloning and polymerase chain reaction. Students with credit for BISC 357 may not take this course for further credit.
This course will introduce students to recombinant nucleic acid methods. Lab time will involve one full afternoon per week of lab work, for which attendance is mandatory—unexcused absences will be penalized. There will be additional open lab time required before and/or after your lab slot to setup and analyze results. Lecture time will cover theory relevant to the lab work.
- Introduction: safety, equipment, microbiology and sterile technique, using micropipettors.
- mCherry Promoter Swap: Plasmid miniprep, restriction digestion, DNA gel electrophoresis, phosphatase treatment, DNA ligation, and plasmid transformation.
- mCherry Colour Change: Site directed mutagenesis.
- Splicing analysis: Preparation and characterization of genomic DNA and total RNA, reverse transcription, PCR, and gel electrophoresis.
- Ribosomal Sequence Analysis: Gel purification of DNA, TOPO TA cloning, DNA sequencing, DNA sequence analysis and alignment.
Experiments are subject to change or rearrangement.
- Lab reports 20%
- Lab notebooks 20%
- Lab performance 20%
- Lab quizzes 10%
- 2 in-class exams 30%
There will be a lab the first week of classes.
MATERIALS + SUPPLIES:
Safety goggles and a lab coat are required.
A laboratory notebook which can be purchased from the Bookstore is required.
Description: LAB BK LAB NOTES BL
Item Number 6547919101
A non-refundable fee of $12.50 will be assesssed for the laboratory manual.
Weaver, R.F. (2012) Molecular Biology, (5th Ed.) McGraw Hill. Students should have this text from having taken MBB 331.
The following text is strongly recommended: Dale, Jeremy W. and Malcolm von Schantz. (2012). From Genes to Genomes and Applications of DNA Technology, (3rd Ed.). Wiley.
Department Undergraduate Notes:
- Students are advised to review the plagiarism tutorial found at
- For help with writing, learning and study strategies please contact the Student Learning Commons at
- Students requiring accommodations as a result of a disability, must contact the Centre for Students with Disabilities (778-782-3112 or e-mail: email@example.com)