Simon Fraser University
Engaging the World

Course Outlines

Fall 2017 - BISC 357 D100

Genetic Engineering (4)

Class Number: 2230

Delivery Method: In Person

Overview

  • Course Times + Location:

    Sep 5 – Dec 4, 2017: Mon, Wed, Fri, 10:30–11:20 a.m.
    Burnaby

  • Exam Times + Location:

    Dec 15, 2017
    Fri, 12:00–3:00 p.m.
    Burnaby

  • Prerequisites:

    BISC 202, MBB 222, and MBB 231, all with a grade of C- or better. Recommended: MBB 331.

Description

CALENDAR DESCRIPTION:

Techniques in gene cloning and the evaluation of gene function are introduced in lectures and practiced in labs. Lab exercises include genomic DNA, RNA, and plasmid purification; PCR; various cloning techniques; and construction of novel genes. Students are introduced to additional techniques in lectures including gene editing, and genome sequencing. Students with credit for MBB 308 may not take this course for further credit.

COURSE DETAILS:

Course Website: CANVAS

There is a demand for people that are skilled in the various techniques associated with genetic engineering. Biomedical companies and institutes regularly search for people with practical experience in genetic engineering. These skills are valued also for an academic career in disciplines ranging from medicine to ecology. First you will learn how to purify DNA and RNA and clone a complementary DNA fragment in a basic plasmid. After that, you will design and generate DNA a DNA construct for CRISPR-based loss-of-function analysis of gene function, followed by Sequence and Ligase Independent Cloning for over expression of genes as well as marker gene based evaluation of where and and when genes are expressed in an organism. The course will also contain a brief introduction to key technologies behind the ongoing revolution in genomics research. Teaching and exams focus on the practical logic of experiments rather than extensive rote memorization. 

Grading

  • Laboratory exercises and report on gene fusion construction 40%
  • Midterm Exam 20%
  • Final Exam 40%

Materials

REQUIRED READING:

Lecture Material

Provided on-line material and links

Provided protocols

RECOMMENDED READING:

Links in provided on-line material

Registrar Notes:

SFU’s Academic Integrity web site http://students.sfu.ca/academicintegrity.html is filled with information on what is meant by academic dishonesty, where you can find resources to help with your studies and the consequences of cheating.  Check out the site for more information and videos that help explain the issues in plain English.

Each student is responsible for his or her conduct as it affects the University community.  Academic dishonesty, in whatever form, is ultimately destructive of the values of the University. Furthermore, it is unfair and discouraging to the majority of students who pursue their studies honestly. Scholarly integrity is required of all members of the University. http://www.sfu.ca/policies/gazette/student/s10-01.html

ACADEMIC INTEGRITY: YOUR WORK, YOUR SUCCESS