Spring 2020 - MBB 308 D100
Molecular Biology Laboratory (3)
Class Number: 2735
Delivery Method: In Person
Modern molecular biological and recombinant nucleic acid methods will be covered. Examples are DNA and RNA isolation, plasmid preparation, restriction enzyme digestion, DNA cloning and polymerase chain reaction. Students with credit for BISC 357 may not take this course for further credit.
This course will introduce students to recombinant nucleic acid methods. Lab time will involve one full afternoon per week of lab work, for which attendance is mandatory—unexcused absences will be penalized. There will be additional open lab time required before and/or after your lab slot to setup and analyze results.
- Introduction: setting up a lab, safety, equipment, microbiology and sterile technique, using micropipettors.
- Preparation and characterization of genomic DNA.
- Plasmid miniprep, restriction digestion, gel electrophoresis.
- Polymerase Chain reaction.
- Isolation of DNA from a gel, subcloning into a plasmid.
- Site-directed mutagenesis.
- DNA sequencing. Computer analysis of DNA sequence data.
Experiments are subject to change or rearrangement.
- Lab assignment 5%
- Lab exam 15%
- Lab notebooks 20%
- Lab performance 20%
- Lab quizzes 10%
- 2 in-class exams 30%
There will be a lab the first week of classes.
MATERIALS + SUPPLIES:
Safety goggles and a lab coat are required.
A non-refundable fee of $12.50 will be assesssed for the laboratory manual.
Dale, Jeremy W. and Malcolm von Schantz. (2012). From Genes to Genomes and Applications of DNA Technology, (3rd Ed.). Wiley.
Department Undergraduate Notes:
- For help with writing, learning and study strategies please contact the Student Learning Commons at
- Students requiring accommodations as a result of a disability, must contact the Centre for Accessible Learning (778-782-3112 or e-mail: firstname.lastname@example.org)
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