Microscopy Equipment

WaveFX Spinning Disc Confocal System (Quorum Technologies)

• Zeiss Axio Observer inverted microscope
• Yokogawa CSU-10 confocal head
• Lasers: 441, 491, 561, 647 nm
• Objectives: 10X, 25X multi-immersion, 40X oil, 63X oil, 63X water/glycerin, 100X oil
• Hammamatsu 9100 EMCCD camera
• Capabilities: FRAP (405 300mW laser); Photoactivation; FRET
• Software: Volocity acquisition, quantitation, visualization, deconvolution; FRAP modules; Improvision License Server
  

Training and Standard Operating Procedures for WaveFX spinning disc confocal system

Nikon A1R Laser Scanning Confocal System

• Nikon Ti inverted microscope
  
• 4 Fluorescent Photomuplier Tubes plus a 32 channel spectral detector
  
• Lasers: 405, 457, 477, 488, 514, 561, and 638 nm
  
• Objectives: 10X, 20X multi-immersion, 40X oil, 60X oil and 60X water
  
• Hammamatsu 9100 EMCCD camera
• Capabilities: FRAP, Photoactivation, FRET and spectral unmixing
  
• Software: Nikon Elements 4.2 acquisition, quantitative measurements, deconvolution
  

Training and Standard Operating Procedures for Nikon A1R laser scanning confocal system

Zeiss Airyscan

• Zeiss Axio Observer inverted microscope
• LSM 880 conventional laser scanning confocal with GaAsP detectors and a spectral detector
  
• Airyscan superresolution detector with fast scan option
  
• 405, 457, 488, 514, 561, 595, and 638 nm lasers
  
• Dry 10x and dry 20x lenses as well as 40x water, 63x oil, 100x oil and 100x TIRF oil lenses
  
• Environmental control chamber for live cell imaging
  
• FRAP, FRET, photoactivation and spectral unmixing capabilities
  
• Image analysis and deconvolution

Training and Standard Operating Procedures for Zeiss Airyscan 

Leica EM ICE and EM AFS2 Systems

For electron microscopy sample preparation, the Leica EM ICE high-pressure freezing system preserves native cellular ultrastructure without chemical fixation. Coupled with the Leica EM AFS2, pressure-frozen samples can be embedded via automated freeze substitution, yielding room-temperature specimens suitable for sectioning and subsequent examination by electron microscopy.