FACS equipment

BD Jazz

• Data acquisition and cell sorting capabilities (2 tubes, 24, 36,...96-well plates, slides)
• Lasers: 488 nm (blue), 640 nm (red)
• Band pass filters
1. Blue laser: 530/30 nm (FITC), 585/29 nm (PE or PI), 692/40 nm (PerCP), 750/LP (PE-Cy7)
2. Red laser: 670/30 nm (APC), 750/LP (APC-Cy7)
• Software: BDsortware for acquisition, FloJo for analysis

Millipore Guava

• Data acquisition only
• Lasers: 488 nm (Blue), 640 nm (Red)
• Band pass filters: 525/30 nm, 583/26 nm, 690/50 nm, 660/19 nm, 785/70 nm

Funding

Funding for the FACS Facility was initially faciliated through a Tier I Canada Research Chair (to J. Scott) and provided by the following National and B.C. agencies, and units at SFU:

• Canadian Foundation for Innovation
• British Columbia Knowledge Development Fund
• Molecular Biology and Biochemistry
• Dean of Science
• NSERC Research tools and Instruments grant (biosafety cabinet)

Location of Facility

The facility is situated in the TASC2 building, room 8130 (directions - click on TASC2).

Useful FACS Resources

• Training and Standard Operating Procedures