Protein Expression, Purification and Crystallization (PEPC) Facility

Systems and Instruments in the PEPC Facility:

ÄKTA Go Protein Purification System: The AKTA Go chromatography system is a compact FPLC system with flow rates up to 25 ml/min and pressures up to 5 MPa, allowing for affinity, size exclusion, and ion exchange chromatography. It is designed for routine protein purification.

AKTA PurifierThe AKTA Purifier is an older version of the AKTA Go FPLC system, having flow rates between 0.01 ml/min to 100 ml/min and pressures up to 5 MPa. It is a modular, automated protein and peptide purification system compatible with a wide range of columns and solvents.

Eppendorf 5810R tabletop centrifugeThe Eppendorf 5810R tabletop centrifuge can maintain temperatures between -9 °C to 40 °C. Available rotors are:

  • F34-6-38 (fixed angle), max speed 12,000 rpm; 18,514 x g max.
  • A-4-62 (swinging bucket), max speed 4,000 rpm; 3220 x g max.
  • A-4-62 (+ MTP buckets for plates), max speed 4,000 rpm; 2,750 x g max.

Some adapters are also available.

Mosquito dispensing robot: The Mosquito is a general purpose dispensing robot capable of precision liquid handling. It has a volume range of 50 nl to 1.2 uL and can pipette with 16 or 8 disposable tips simultaneously to fill a 384-well plate-to-plate copy in less than 3 minutes with zero cross contamination. The Mosquito software can be programmed to perform multiple pipetting operations including volume settings, plate types, and plate positions.

Fraction collector F9-TThe fraction collector F9-T, associated with the AKTA Go system, enables fraction collection in deep-well plates, 96 well plates, or tubes.

Max Q 8000 ShakersThe Max Q8000 digital, orbital incubator/shaker can hold 10 2L flasks, shake between 25-400 rpm, and hold temperatures from -5 °C below ambient or more reliably, 20 °C to 60 °C. This shaker is for culture of bacteria.


Training is required for first time users, please contact Lab Operation Manager for arrangement for training and access.  Training will be conducted by a member of the PEPC team: King or Craig lab for the Mosquito, and Craig lab for the AKTA Go. 


PEPC online booking

Fees For academic users:

(Other users, please contact Lab Operation Manager for rates.)

Mosquito Crystallization Robot:

General users: $52/plate (x 2, one for the JCSG-plus screen, one for the NeXtal PACT screen, 192 conditions total) 

Users who supply their own screens: $45/plate

AKTA Go Protein Purification FPLC: 

$50 for 12 hr, $75 for 24 hr


$20/24 hr


Traning sessions are charged as: $104 for Mosquito Robot and $150 for AKTA FPLC. 


MBB Department


PEPC online booking


Manager, Laboratory Operations

 (604) 518-9066