Optima L-90K floor model ultracentrifuge

The Beckman Optima L-90K floor model ultracentrifuge is taken care of by the MBB Manager, Laboratory Operations. All users must contact the Manager (details below) to receive training and gain access to the rotors which are stored in a locked location. Users must supply their own tubes and they must be the tubes specified by Beckman for each rotor. Each rotor manual has a page listing the acceptable tubes that can be used.

WARNING: CENTRIFUGES AND ULTRACENTRIFUGES CAN BE DANGEROUS. ALWAYS:

1) Receive training on the use of the machine.

2) Use the correct rotor with the correct machine.

3) Use the correct tubes with the correct rotor.

4) Do NOT under or overfill the tubes.

4) Balance the tubes to within 0.05-0.1g of each other.

5) Balance the load in the rotor.

6a) Place the rotor correctly on the centrifuge spindle.

6b) Know the maximum speed of the tubes, the rotor, and the centrifuge. They may not be the same.

7) Do NOT spin at or close to the maximum speed. 

8) Stay with the centrifuge until full operating speed is reached. If there are unusual noises or vibrations, shut the machine down ASAP.

9) When the run is finished, remove the rotor, check that it is clean and dry.

10) Report any concerns to the MBB Manager, Laboratory Operations.

User manual (equipment)

Short instructions

User manual (Ti-70 rotor)  

User manual (Ti 90 rotor)   

The Beckman Optima L-90K floor model ultracentrifuge is a high speed, preparative ultracentrifuge which can be used for applications such as the isolation of plasmid DNA and subcellular particles. The floor model L-90K has temperature control, vacuum, program memory that can contain up to nine programs, and a choice of max or slow acceleration and deceleration rates.

Please book through MBB Instrument Booking

Location

MBB Department

Training must be obtained from the the MBB Manager, Laboratory Operations.

Contact

Manager, Laboratory Operations

Phone:
 (778) 782-4318
E-mail: deidre@sfu.ca