NanoDrop 3300 Fluorospec

The NanoDrop 3300 fluorospectrophotometer is taken care of by the MBB Manager, Laboratory Operations. The 3300 has an associated computer so in order to use the spec, please contact the Manager (details below) to gain access via SFU ID and password.

User Manual (equipment)


The NanoDrop 3300 is a microvolume, broad spectrum fluorospectrophotometer with pedestal. Its small sample requirement and ease of use makes the instrument well suited for a diverse range of fluorescence measurements from RiboGreen, PicoGreen, and GFP to advanced nanoparticle and FRET analyses.

Specifications include:

  • 400 - 750 nm wavelength measurement range.
  • 1 - 2 ul sample volume.
  • Has 3 LEDS. Excitation maxima are: UV: 365 nm; Blue: 470 nm; White: 460 – 650 nm.
  • UV LEDmax = 365 nm; equipped with cut filter that eliminates excitation above 400 nm | Example Applications: GFP wt, Hoechst 33258, 4-MU, Q Dots various, Fluoraldehyde OPA, Fluorescamine, FRET.
  • Blue LEDmax = 470 nm | Example Applications: GFP wt, eGFP, FITC-FAM, Alexa 488, PicoGreen, RiboGreen, Alexa 555, B-Phycoerythrin, Q Dot various, SybrGreen, SybrGold, FRET.
  • White LED range = 460 – 650 nm; uses virtual filtering | Example Applications: Cy3, Alexa 555, Alexa 568, Cy5, Alexa 647, Sulforhodamine 101, 5-CMTR, Q Dots various, TET, Hex, Molecular beacons.

Detailed Specs

No Booking Required


MBB Department

Training must be obtained from the MBB Manager, Laboratory Operations.


Manager, Laboratory Operations

 (778) 782-4318