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- Congratulations to Dr. Amy Lee: The Recipient of The Banting Research Foundation's 2022 Discovery Award
- Meet Our Newest Faculty: Dr. Dustin King on His Exploration of Weaving Western Science with Indigenous Ways of Knowing
- MBB Alumnus Profile: Meet Cory Macklin Who Overcame Challenges to Win Governor General's Silver Medal
- MBB Alumnus Profile: Meet Dr. Razvan Cojocaru, The Recipient of Governor General’s Gold Medal
- Congratulations to Dr. Razvan Cojocaru and Cory Macklin: Governor General Medal Recipients at Summer 2022 Convocation
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- Dr. Lynne Quarmby and Her Book "WaterMelon Snow: Science, Art, and a Lone Polar Bear" is Featured in SFU Knowledge Mobilizers Series
- Congratulations to our May 2022 Graduands
- Congratulations to Dr. Mani Larijani for Being Awarded a Prestigious New Frontiers in Research Fund Grant
- A New Paper from Beh Lab on ER-PM membrane contact site regulation by yeast ORPs and membrane stress pathways has been published in Plos Genetics Journal
- Congratulations to Dr. Razvan Cojocaru, the recipient of Dr. Bruce Brandhorst Prizes for Best Publication and Best PhD Thesis
- Dr. Lorena Braid is featured among new and renewed Canada Research Chairs
- Learn more about Dr. King's new lab that focuses on natural ‘carbon capture’ solutions
- $2 Million Gift From SFU Professors Emeriti Helps Seed New Ideas at SFU Science
- Congratulations to Dr. Tim Audas on The Renewal of Tier 2 Canada Research Chair in Cellular Stress
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ABI StepOne RT PCR Machine
The Applied Biosystems StepOne RT PCR machine is taken care of by the MBB Manager, Laboratory Operations. The StepOne has an associated computer so in order to use the PCR machine, please contact the Manager (details below) to gain access via SFU ID and password.
User guides for the StepOne RT PCR machine:
The ABI StepOne RT PCR machine is a 48-well, low-throughput Real Time PCR machine.
Specifications:
- 3-colour optical LED recording system; records fluorescence from FAM/SYBR Green, VIC/JOE, and ROX dyes.
- Supports RT PCR applications including:
- SNP genotyping
- Gene expression analysis
- MicroRNA expression
- Translocation analysis
- Gene detection
- Viral load analysis
- Software provides the following analysis methods:
- Standard curve (absolute quantitation)
- Relative standard curve
- Comparative Ct (relative quantitation)
- Genotyping and presence/absence
- Melt curve analysis
Please book through MBB Instrument Booking
Location
MBB Department
Training must be obtained from the MBB Manager, Laboratory Operations.
Contact
Manager, Laboratory Operations
Phone: (778) 782-4318
E-mail: deidre@sfu.ca