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- Nikon Ti inverted microscope
- 4 Fluorescent Photomuplier Tubes plus a 32 channel spectral detector
- Lasers: 405, 457, 477, 488, 514, 561, and 638 nm
- Objectives: 10X, 20X multi-immersion, 40X oil, 60X oil and 60X water
- Hammamatsu 9100 EMCCD camera
- Capabilities: FRAP, Photoactivation, FRET and spectral unmixing
- Software: Nikon Elements 4.2 acquisition, quantitative measurements, deconvolution
Training and Standard Operating Procedures for Nikon A1R laser scanning confocal system
Technical Support
Contact
TIM HESLIP
Microscopy Technician
Email: mbb_microscopy@sfu.ca
Office: B 7145
Tel: (778) 782-6807
Availability Schedule
Tue-Thu 9:00AM-4:30PM
Users are charged for time booked unless they cancel 6 hours in advance or find an alternate user.
Fee Schedule
Instrument Type |
SFU Users
|
SFU Users
|
Other BC Universities
|
Non-SFU Users |
| WaveFX/Nikon | $10/hr | $5/hr | $15/hr | $20/hr |
| Airscan | $25/hr | $12.50/hr | $30/hr | $50/hr |
| Leica EM ICE (HP Freezer) | $50/day* | - | $150/day* | $150/day* |
| Leica EM ICE AFS2 | $50/day* | - | $50/day* | $50/day* |
| Training | $50/hr | - | $50/hr | $100/hr |
*Liquid nitrogen is required to run both instruments. Users will be charged $80/100 L in addition to the user charge.
Resources
Useful Microscopy Resources
- Fluorescence SpectraViewer from ThermoFi
- Volocity software information
Microscopy Oversight Committee
Name |
Tel |
|
| Tim Audas (Chair) | taudas@sfu.ca | (778) 782-6811 |
| Christopher Beh | ctbeh@sfu.ca | (778) 782-6801 |
| Valentin Jaumouillé | vjaumoui@sfu.ca | (778) 782-5682 |
| Michel Leroux | leroux@sfu.ca | (778) 782-6683 |
Lens Cleaning Policy
It is the responsibility of every user to inspect the objective lenses and the rest of the microscope and anti-vibration table for oil or other waste. Any problem needs to be noted on the log and the Microscopy Technician notified immediately. Failure to report problems could result in the most recent user on the log to be held responsible. It is the responsibility of every user to check and see if they are the last user of the day. This must be done using the Nikon Confocal calendar on the MBB Facilities web site. Failure to power down the Nikon Confocal overnight will result in disciplinary action.
Actions for not properly cleaning lenses and failure to power down
1st offence: Verbal discussion
2nd offence: Email user and their supervisor plus the Microscopy Oversight Committee and the Chair of MBB
3rd offence: User’s supervisor is charged $100 and the user is notified that they can potentially be banned from use.
4th offence: The user is banned from using the confocal until further notice.
Data Storage Policy
The confocal acquisition computers are not to be used for data storage. Backup your images at the end of each session and then delete from the acquisition computer hard drive at the soonest possible opportunity. Images not deleted from the acquisition computers can be deleted without notice at any time if needed to create free hard drive space.
Funding
This facility has been generously funded by the Canadian Foundation for Innovation, NSERC, British Columbia Knowledge Development Fund, Molecular Biology and Biochemistry, Biological Sciences, Office of the Vice President Research and Simon Fraser University.