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- Zeiss Axio Observer inverted microscope
- LSM 880 conventional laser scanning confocal with GaAsP detectors and a spectral detector
- Airyscan superresolution detector with fast scan option
- 405, 457, 488, 514, 561, 595, and 638 nm lasers
- Dry 10x and dry 20x lenses as well as 40x water, 63x oil, 100x oil and 100x TIRF oil lenses
- Environmental control chamber for live cell imaging
- FRAP, FRET, photoactivation and spectral unmixing capabilities
- Image analysis and deconvolution
Training and Standard Operating Procedures for Zeiss Airyscan
Technical Support
Contact
TIM HESLIP
Microscopy Technician
Email: mbb_microscopy@sfu.ca
Office: B 7145
Tel: (778) 782-6807
Availability Schedule
Tue-Thu 9:00AM-4:30PM
Users are charged for time booked unless they cancel 6 hours in advance or find an alternate user.
Fee Schedule
Instrument Type |
SFU Users
|
SFU Users
|
Other BC Universities
|
Non-SFU Users |
| WaveFX/Nikon | $10/hr | $5/hr | $15/hr | $20/hr |
| Airscan | $25/hr | $12.50/hr | $30/hr | $50/hr |
| Leica EM ICE (HP Freezer) | $50/day* | - | $150/day* | $150/day* |
| Leica EM ICE AFS2 | $50/day* | - | $50/day* | $50/day* |
| Training | $50/hr | - | $50/hr | $100/hr |
*Liquid nitrogen is required to run both instruments. Users will be charged $80/100 L in addition to the user charge.
Resources
Useful Microscopy Resources
- Fluorescence SpectraViewer from ThermoFi
- Volocity software information
Images - AiryScan

Cultured neural stem cells (green; PAX6) differentiating into newborn neurons (red; β-tubulin). DNA (blue). (L. Julian)

U2OS osteosarcoma cells stained for TOPBP1 (green), CIP2A (red) and DNA (blue). (D. Setiaputra)

Cardiomyocytes derived from human induced pluripotent stem cells. α-actinin in Z-discs, green; mitochondria, red; DNA, blue. (G. Tibbits)

Intraflagellar transport motors Kin-II (green) and OSM-3 (red) in C. elegans cilia. (M. Leroux)
Microscopy Oversight Committee
Name |
Tel |
|
| Tim Audas (Chair) | taudas@sfu.ca | (778) 782-6811 |
| Christopher Beh | ctbeh@sfu.ca | (778) 782-6801 |
| Valentin Jaumouillé | vjaumoui@sfu.ca | (778) 782-5682 |
| Michel Leroux | leroux@sfu.ca | (778) 782-6683 |
Lens Cleaning Policy
It is the responsibility of every user to inspect the objective lenses and the rest of the microscope and anti-vibration table for oil or other waste. Any problem needs to be noted on the log and the Microscopy Technician notified immediately. Failure to report problems could result in the most recent user on the log to be held responsible. It is the responsibility of every user to check and see if they are the last user of the day. This must be done using the Nikon Confocal calendar on the MBB Facilities web site. Failure to power down the Nikon Confocal overnight will result in disciplinary action.
Actions for not properly cleaning lenses and failure to power down
1st offence: Verbal discussion
2nd offence: Email user and their supervisor plus the Microscopy Oversight Committee and the Chair of MBB
3rd offence: User’s supervisor is charged $100 and the user is notified that they can potentially be banned from use.
4th offence: The user is banned from using the confocal until further notice.
Data Storage Policy
The confocal acquisition computers are not to be used for data storage. Backup your images at the end of each session and then delete from the acquisition computer hard drive at the soonest possible opportunity. Images not deleted from the acquisition computers can be deleted without notice at any time if needed to create free hard drive space.
Funding
This facility has been generously funded by the Canadian Foundation for Innovation, NSERC, British Columbia Knowledge Development Fund, Molecular Biology and Biochemistry, Biological Sciences, Office of the Vice President Research and Simon Fraser University.