- About
- People
- Faculty
- Tim Audas
- Christopher Beh
- Lorena Braid
- Fiona S.L. Brinkman
- Mark Brockman
- Tom Cech
- Jonathan Choy
- Lisa Craig
- Sharon Gorski
- Nicholas Harden
- Nancy Hawkins
- Robert Holt
- William Hsiao
- Valentin Jaumouillé
- Lisa Julian
- Dustin King
- Irina Kovalyova
- Mani Larijani
- Amy Lee
- Michel Leroux
- Ryan Morin
- Ingrid Northwood
- Mark Paetzel
- Lynne Quarmby
- Dheva Setiaputra
- Michael Silverman
- Sophie Sneddon
- Peter Unrau
- Esther Verheyen
- Stephanie Vlachos
- David Vocadlo
- Edgar Young
- Emeritus Faculty
- Associate Members
- Adjunct Faculty
- Research Personnel
- Graduate & Postdocs
- Staff
- Department Committees
- Faculty
- Undergraduate
- Prospective Students
- Admission & Transferring
- Degree Programs
- MBB Co-op Program
- Careers in Molecular Biology and Biochemistry
- Scholarships & Funding
- Student Stories
- Contact Us
- Current Students
- Advising
- Courses
- Degree Programs
- MBB Co-op Program
- Research Opportunities
- Scholarship & Funding
- Student Resources
- Courses
- Prospective Students
- Graduate
- Graduate Studies Overview and Timeline
- Prospective Students
- Graduate Programs
- Degree Requirements
- Application Process
- Courses
- Course Descriptions
- Course Offerings
- Spring (1261)
- Spring (1251)
- Summer (1254)
- Fall (1257)
- Spring (1241)
- Summer (1244)
- Fall (1247)
- Spring (1231)
- Summer (1234)
- Fall (1237)
- Spring (1221)
- Summer (1224)
- Fall (1227)
- Fall (1217)
- Summer (1214)
- Spring (1211)
- Fall (1207)
- Summer (1204)
- Spring (1201)
- Fall (1197)
- Summer (1194)
- Spring (1191)
- Fall (1187)
- Summer (1184)
- Spring (1181)
- Fall (1177)
- Summer (1174)
- Spring (1171)
- Fall (1167)
- Summer (1164)
- Spring (1161)
- Graduate Student Stipend & Awards
- Events
- Student Stories
- Grad Student Opportunities
- FAQs
- Current Students
- Manage Your Program
- Degree Requirements
- Courses
- Course Descriptions
- Course Offerings
- Spring (1261)
- Spring (1251)
- Summer (1254)
- Fall (1257)
- Spring (1241)
- Summer (1244)
- Fall (1247)
- Spring (1231)
- Summer (1234)
- Fall (1237)
- Spring (1221)
- Summer (1224)
- Fall (1227)
- Fall (1217)
- Summer (1214)
- Spring (1211)
- Fall (1207)
- Summer (1204)
- Spring (1201)
- Fall (1197)
- Summer (1194)
- Spring (1191)
- Fall (1187)
- Summer (1184)
- Spring (1181)
- Fall (1177)
- Summer (1174)
- Spring (1171)
- Fall (1167)
- Summer (1164)
- Spring (1161)
- Fall (1157)
- Spring (1151)
- Graduate Student Stipend & Awards
- Forms And Resources
- Events
- Graduate Student Caucus
- Research
- Research Labs
- Audas Lab
- Beh Lab
- Braid lab
- Brinkman Lab
- Brockman Lab
- Choy Lab
- Craig Lab
- Gorski Lab
- Hawkins Lab
- Holt Lab
- Hsiao Lab
- Jaumouillé Lab
- Julian Lab
- King Lab
- Larijani Lab
- Lee Lab
- Leroux Lab
- Morin Lab
- Paetzel Lab
- Quarmby Lab
- Sen Lab
- Setiaputra Lab
- Silverman Lab
- Tibbits Lab
- Unrau Lab
- Verheyen Lab
- Vocadlo Lab
- Young Lab
- Bioinformatics & Genomics
- Cells & Disease
- Infection & Immunity
- Macromolecular Biochemistry
- Undergraduate Research Opportunities
- Postdoctoral Research Opportunities
- C2D2 Centre for Cell Biology, Development, and Disease
- Omics Data Science Initiative
- Recent Publications
- Research Labs
- Resources
- News & Events
- Support MBB
- Faculty + Staff Portal
- Zeiss Axio Observer inverted microscope
- Yokogawa CSU-10 confocal head
- Lasers: 441, 491, 561, 647 nm
- Objectives: 10X, 25X multi-immersion, 40X oil, 63X oil, 63X water/glycerin, 100X oil
- Hammamatsu 9100 EMCCD camera
- Capabilities: FRAP (405 300mW laser); Photoactivation; FRET
- Software: Volocity acquisition, quantitation, visualization, deconvolution; FRAP modules; Improvision License Server
Training and Standard Operating Procedures for WaveFX spinning disc confocal system
Technical Support
Contact
TIM HESLIP
Microscopy Technician
Email: trh2@sfu.ca
Office: B 7145
Tel: (778) 782-6807
Availability Schedule
Tue-Thu 9:00AM-4:30PM
Users are charged for time booked unless they cancel 6 hours in advance or find an alternate user.
Fee Schedule
Instrument Type |
SFU Users
|
SFU Users
|
Other BC Universities
|
Non-SFU Users |
| WaveFX/Nikon | $10/hr | $5/hr | $15/hr | $20/hr |
| Airscan | $25/hr | $12.50/hr | $30/hr | $50/hr |
| Leica EM ICE (HP Freezer) | $50/day* | - | $150/day* | $150/day* |
| Leica EM ICE AFS2 | $50/day* | - | $50/day* | $50/day* |
| Training | $50/hr | - | $50/hr | $100/hr |
*Liquid Nitrogen costs $80 per 100L (N2 required to run both instruments) in addition to the user charge.
Resources
Useful Microscopy Resources
- Fluorescence SpectraViewer from ThermoFi
- Volocity software information
Microscopy Oversight Committee
Name |
Tel |
|
| Christopher Beh | ctbeh@sfu.ca | (778) 782-6801 |
| Valentin Jaumouillé | vjaumoui@sfu.ca | (778) 782-5682 |
| Michel Leroux | leroux@sfu.ca | (778) 782-6683 |
| Tim Audas | taudas@sfu.ca | (778) 782-6811 |
Lens Cleaning Policy
It is the responsibility of every user to inspect the objective lenses and the rest of the microscope and anti-vibration table for oil or other waste. Any problem needs to be noted on the log and the Microscopy Technician notified immediately. Failure to report problems could result in the most recent user on the log to be held responsible. It is the responsibility of every user to check and see if they are the last user of the day. This must be done using the Nikon Confocal calendar on the MBB Facilities web site. Failure to power down the Nikon Confocal overnight will result in disciplinary action.
Actions for not properly cleaning lenses and failure to power down
1st offence: Verbal discussion
2nd offence: Email user and their supervisor plus the Microscopy Oversight Committee and the Chair of MBB
3rd offence: User’s supervisor is charged $100 and the user is notified that they can potentially be banned from use.
4th offence: The user is banned from using the confocal until further notice.
Data Storage Policy
The confocal acquisition computers are not to be used for data storage. Backup your images at the end of each session and then delete from the acquisition computer hard drive at the soonest possible opportunity. Images not deleted from the acquisition computers can be deleted without notice at any time if needed to create free hard drive space.
Funding
This facility has been generously funded by the Canadian Foundation for Innovation, NSERC, British Columbia Knowledge Development Fund, Molecular Biology and Biochemistry, Biological Sciences, Office of the Vice President Research and Simon Fraser University.